A Laboratory Guide to the Tight Junction

Download or read online book entitled A Laboratory Guide to the Tight Junction written by Jianghui Hou and published by Academic Press. This book was released on 02 August 2020 with total page 416 pages. Available in PDF, EPUB and Kindle. Get best books that you want by click Get Book Button and Read as many books as you like. Book Excerpt : A Laboratory Guide to the Paracellular Channel offers broad coverage of the unique methods required to investigate its characteristics. Methods are described in detail, including biochemical and biophysical principles, step-by-step processes, data analysis, troubleshooting and optimization. Coverage includes various cell, tissue and animal models. Specific sections cover the foundations of ion transport across cell membrane and tight junction, biochemical approaches for paracellular channels, fixation approaches, tissue preparation, light microscopy, the electron microscopy approach, transgenic manipulation, including DNA and siRNA, mutagenesis, retroviral, lentiviral, and adenoviral infection, and much more. Researchers and advanced students in bioscience working to understand the intercellular channel will benefit from the detailed methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical foundations of paracellular channels and their connection to disease states.